Journal: Brain : a journal of neurology

Article Title: Connexin43 mimetic peptide reduces vascular leak and retinal ganglion cell death following retinal ischaemia.

doi: 10.1093/brain/awr338

Figure Lengend Snippet: Figure 3 Three hours of hypoxia and 6 h of reperfusion lead to significant endothelial cell death in vitro in rat brain microvascular endothelial cells (RBMVEC; A). Non-specific gap junction blocker carbenoxolone, non-specific hemichannel blocker LaCl3, and connexin43 (Cx43) mimetic peptide protected endothelial cells against hypoxic injury, with the number of viable cells significantly higher than no treatment, while scrambled peptide did not have any protective effects. The number of viable cells was expressed as percentage of the control without hypoxia. Hypoxia and reperfusion also lead to significant propidium iodide dye uptake into primary rat brain microvascular endothelial cells indicating open hemichannels (B). Carbenoxolone, LaCl3 and connexin43 mimetic peptide significantly prevented dye uptake compared to no treatment, indicating hemichannel closure, while scrambled peptide did not have any effect. Stars denote statistical significance when compared to the control group or compared between groups in brackets; P 5 0.05.

Article Snippet: Rat brain microvascular endothelial cells (R840K-05a, Cell Applications) were plated into 24-well plates (1 105 cells/well) in rat brain microvascular endothelial cell growth media (R819K-500, Cell Applications) and allowed to settle for 16 h. Medium was then removed and replaced with Dulbecco’s Modified Eagle’s Medium/F12 containing 0.5% foetal bovine serum and 1% glutamine.

Techniques: In Vitro, Control

Journal: International Journal of Molecular Sciences

Article Title: Generation of Mesenchymal Cell Lines Derived from Aged Donors

doi: 10.3390/ijms221910667

Figure Lengend Snippet: Relative expression levels (RELs) of Runx2, Sox9 and Col10A1 in iMSCs spheroids at the beginning of the experiment (t = 0) and after 21 days of chondrogenic induction (t = 21). RELs of each gene were scaled to the REL of the same gene in primary chondrocyte spheroids. Data obtained from iMSC#6, iMSC#9 and iMSC#10 spheroids were employed to calculate the mean values shown.

Article Snippet: Both hanging drop method and pellet cultures were employed to create cell aggregates, which were subsequently incubated in hMSC Chondrogenic Differentiation Medium supplemented with 10 ng/mL of human transforming growth factor β-3 (TGF-β3) (ProSpec-Tany TechnoGene, Rejovot, Israel) for 21 days.

Techniques: Expressing

Journal: Biotechnology and Bioengineering

Article Title: Expansion, harvest and cryopreservation of human mesenchymal stem cells in a serum‐free microcarrier process

doi: 10.1002/bit.25582

Figure Lengend Snippet: Nutrient and metabolite flux of hMSC expansion on microcarriers. Glucose, lactate and ammonia concentrations in FBS‐containing medium (A) and serum‐free medium (B). Total protein (C) and lactate dehydrogenase concentration (D) are shown for FBS‐containing and serum‐free medium. Data shows mean ± SD, n = 3.

Article Snippet: The hMSC differentiation was induced using PRIME‐XV TM hMSC Differentiation Medium (Irvine Scientific, USA) as per manufacturer's instructions.

Techniques: Concentration Assay

Journal: Biotechnology and Bioengineering

Article Title: Expansion, harvest and cryopreservation of human mesenchymal stem cells in a serum‐free microcarrier process

doi: 10.1002/bit.25582

Figure Lengend Snippet: Post‐harvest hMSC quality compared to pre‐expansion demonstrating retention of key attributes, showing (A) specific growth rate, (B) colony forming efficiency, (C) mean cell diameter and (D) forward/side scatter of cell populations confirming difference in mean cell diameter.

Article Snippet: The hMSC differentiation was induced using PRIME‐XV TM hMSC Differentiation Medium (Irvine Scientific, USA) as per manufacturer's instructions.

Techniques:

Journal: Biotechnology and Bioengineering

Article Title: Expansion, harvest and cryopreservation of human mesenchymal stem cells in a serum‐free microcarrier process

doi: 10.1002/bit.25582

Figure Lengend Snippet: Post‐expansion harvest of hMSCs from microcarriers showing successful hMSC detachment from microcarriers (A). Post‐harvest viability shows high number of intact hMSCs for FBS‐containing medium and serum‐free medium. Data shows mean ± SD, n = 3.

Article Snippet: The hMSC differentiation was induced using PRIME‐XV TM hMSC Differentiation Medium (Irvine Scientific, USA) as per manufacturer's instructions.

Techniques:

Journal: Biotechnology and Bioengineering

Article Title: Expansion, harvest and cryopreservation of human mesenchymal stem cells in a serum‐free microcarrier process

doi: 10.1002/bit.25582

Figure Lengend Snippet: Post‐harvest hMSC characterisation. (A) pre‐expansion and (B) post‐harvest hMSC morphology in FBS‐containing medium. (C) pre‐expansion and (D) post‐harvest hMSC morphology in serum‐free medium. Tri‐lineage differentiation of hMSCs showing (E) osteogenic, (F) adipogenic and (G) chondrogenic potential post‐harvest in serum‐free medium. Multiparameter flow cytometry showing dual gating of CD73, 90, 105, 34 and HLA‐DR for hMSCs post‐harvest from serum‐free microcarrier culture (H).

Article Snippet: The hMSC differentiation was induced using PRIME‐XV TM hMSC Differentiation Medium (Irvine Scientific, USA) as per manufacturer's instructions.

Techniques: Flow Cytometry

Journal: Biotechnology and Bioengineering

Article Title: Expansion, harvest and cryopreservation of human mesenchymal stem cells in a serum‐free microcarrier process

doi: 10.1002/bit.25582

Figure Lengend Snippet: Post‐thaw hMSC recovery following serum‐free cryopreservation, showing (A) post‐thaw recovery and 3 h cell attachment based on PI exclusion. Post‐thaw hMSC outgrowth (B) following serum‐free cryopreservation. Data shows mean ± SD (n = 3).

Article Snippet: The hMSC differentiation was induced using PRIME‐XV TM hMSC Differentiation Medium (Irvine Scientific, USA) as per manufacturer's instructions.

Techniques: Cell Attachment Assay

Journal: Biotechnology and Bioengineering

Article Title: Expansion, harvest and cryopreservation of human mesenchymal stem cells in a serum‐free microcarrier process

doi: 10.1002/bit.25582

Figure Lengend Snippet: Post‐thaw hMSC recovery following serum‐free cryopreservation, demonstrating formation of F‐Actin cytoskeleton (A) 3 h, (B) 24 hours post thaw (C) 3 h post‐passage control and (D) 24 hours post‐passage control. Phase contrast images show day 2 hMSC morphology post‐thaw (E) and post passage control (F). Scale bar = 250 μm.

Article Snippet: The hMSC differentiation was induced using PRIME‐XV TM hMSC Differentiation Medium (Irvine Scientific, USA) as per manufacturer's instructions.

Techniques: Control

Journal: Micromachines

Article Title: 3D Printed Wavy Scaffolds Enhance Mesenchymal Stem Cell Osteogenesis

doi: 10.3390/mi11010031

Figure Lengend Snippet: ( A ) Optical microscopy images of the hMSCs stained for alizarin red (red) after culture in osteogenic induction media for 21 days. Scale bars are 200 microns. ( B ) Alizarin red concentration indicating calcium deposition at Day 21. (* p < 0.15, ** p < 0.05, for n = 3).

Article Snippet: For osteogenic differentiation studies, growth media was replaced with osteogenic induction media (hMSC osteogenic differentiation medium BulletKitTM, Lonza, Basel, Switzerland) at Day 7, and cells were cultured for an additional 14 days.

Techniques: Microscopy, Staining, Concentration Assay

Journal: Micromachines

Article Title: 3D Printed Wavy Scaffolds Enhance Mesenchymal Stem Cell Osteogenesis

doi: 10.3390/mi11010031

Figure Lengend Snippet: Multiphoton confocal images for hMSCs that were cultured in osteogenic induction media for 14 ( top row) and 21 days ( bottom row). Cells were immunostained for osteocalcin (green) and stained for F-actin (red) and cell nuclei (blue). Scale bars are 200 microns.

Article Snippet: For osteogenic differentiation studies, growth media was replaced with osteogenic induction media (hMSC osteogenic differentiation medium BulletKitTM, Lonza, Basel, Switzerland) at Day 7, and cells were cultured for an additional 14 days.

Techniques: Cell Culture, Staining